[ Mahidol University. Journal of Pharmaceutical Sciences - ONLINE ]
[ By Faculty of Pharmacy, Mahidol University. THAILAND]
Mahidol University Journal of Pharmaceutical Sciences
Abstracted/Indexed in SciFinder since 1974 (former name  "Warasan Phesatchasat")
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The Mahidol University Journal of Pharmaceutical Sciences is committed to the timely publication of innovative papers and reviews. Manuscripts submitted for publication in this journal must not have been previously published and are not under consideration for publication in any other media. All manuscripts accepted for publication are peer-reviewed... read more


Development of Chemometric Tool for Determination of Peptide Samples Comparing with High-Performance Liquid Chromatography

C. M. Phechkrajang*, S. Jarusintanakorn and O. Vajragupta

In this study, a chemometric tool, partial least square regression (PLS-1), was developed for quantitative determination of synthetic peptide samples, L-alanyl-L-tryptophan (AT) and glycyl-L-phenylalanine in the presence of dansyl-L-phenylalanine. Two sets of mixture solutions, calibration and test sets, were prepared and measured for their UV absorption. The absorbance data of calibration set and the Unscrambler® program were employed for PLS-1 models set up. The obtained models were validated by determination the test set solutions which were not contributed in model construction. On the other hand, a high- performance liquid chromatography (HPLC) method was developed and validated. In comparison, the developed HPLC method was used to determine the same test set as PLS-1 models. By using t-test statistical evaluation, the results obtained from two methods, PLS-1 and HPLC, were found comparable and statistical comparison showed no significant difference at 95% confident interval, indicated replacement ability of PLS-1 to a separation method, HPLC for the determination of peptide samples.

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