[ Mahidol University. Journal of Pharmaceutical Sciences - ONLINE ]
[ By Faculty of Pharmacy, Mahidol University. THAILAND]
Mahidol University Journal of Pharmaceutical Sciences
Abstracted/Indexed in SciFinder since 1974 (former name  "Warasan Phesatchasat")


Development and validation of chemometrics-assisted spectrophotometric method for determination of clotrimazole in the presence of betamethasone valerate

C.M. Phechkrajang*, W. Siriratawan, K. Narapanich, K. Thanomchat, P. Kantanawat,W. Srikajhondei, V. Khajornvanitchot and K. Sakchaisri

A simple and accurate chemometrics-assisted spectrophotometric method was developed for determination of clotrimazole in the presence of betamethasone valerate. Partial-least square method (PLS-1) was selected for this objective. The PLS-1 models of clotrimazole and betamethasone valerate were separately developed on Unscrambler? program by using leave-one-out cross-validation of the calibration set samples. The resulted models were proved for their prediction abilities by using the test set samples, which were not contributed in models building step. The models were also validated for linearity, accuracy, and precision. The validation results showed that all of the parameters were within the acceptable limit. Finally, the developed and validated PLS-1 model was applied to quantitate clotrimazole in vaginal inserts. The determination results were appreciated and highly precised. Therefore, the PLS-1 model may be a simple and accurate alternative method to determine the amount of betamethasone and clotrimazole in formulations containing mixture of them.

DOI: 10.14456/mujps.2015.2

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