[ Mahidol University. Journal of Pharmaceutical Sciences - ONLINE ]
[ By Faculty of Pharmacy, Mahidol University. THAILAND]
Mahidol University Journal of Pharmaceutical Sciences
Abstracted/Indexed in SciFinder since 1974 (former name  "Warasan Phesatchasat")
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The Mahidol University Journal of Pharmaceutical Sciences is committed to the timely publication of innovative papers and reviews. Manuscripts submitted for publication in this journal must not have been previously published and are not under consideration for publication in any other media. All manuscripts accepted for publication are peer-reviewed... read more


Detection of Hemolysin BL Gene of Bacillus cereus Isolates

C. Wiwat* and R. Thiramanas

Hemolysin BL (HBL) is the multi-component enterotoxin causing diarrheal type of Bacillus cereus food poisoning. The hbl operon of B. cereus was divided into 4 coding sequences (CDS) and identified as hblC, hblD, hblA and hblB genes consisting of 1,319, 1,221, 1,128 and 1,401 nucleotides encoding proteins of 439, 406, 375 and 466 amino acids with predicted sizes of 49.3, 43.8, 41.7 and 52.3 kDa, respectively. One hundred of B. cereus isolates were detected for the presence of each hbl gene by multiplex PCR and HBL production by discontinuous hemolysis on HBL sheep blood agar and Bacillus cereus Enterotoxin-Reverse Passive Latex Agglutination (BCET-RPLA) test kit. The results showed that 47 of 100 B. cereus isolates (47%) were detected by multiplex PCR with all hbl genes and gave positive result with RPLA test. Forty-one of 47 isolates (87.2%) produced discontinuous hemolysis on HBL sheep blood agar. However, this pattern was not observed clearly in 6 of 47 isolates (12.8%). In addition, B. cereus isolate E82/44 gave multiplex PCR positive with only 3 hbl genes, hblA, hblC and hblD, also showed positive result with RPLA test and produced discontinuous hemolysis.

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MUPY cordially welcomed Professor Dr. P.T. Thomas from Taylorís University, Malaysia
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